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Books recommended

Biochemistry (Biochemistry (Berg)) by Jeremy M. Berg, John L. Tymoczko, and Lubert Stryer (Hardcover - May 19, 2006)

Lehninger Principles of Biochemistry by David L. Nelson and Michael M. Cox (Hardcover - Feb 1, 2008)

The Biochemistry of the Nucleic Acids by R.L. Adams, J.T. Knowler, and D.P. Leader (Paperback - May 31, 1992)

Principles of Nucleic Acid Structure by Stephen Neidle (Kindle Edition - Oct 5, 2007)

Molecular Biology by Robert Weaver (Paperback - Feb 1, 2007)

Molecular Biology of the Gene (6th Edition) by James D. Watson, Tania A. Baker, Stephen P. Bell, and Alexander Gann (Hardcover - Dec 15, 2007)

David Freifelder (1983) Jones & Bartlett publishers. 2ed Molecular biology. Reprint (1993) Narosa Publishing House.




Title of the paper

PRACTICALS III – PAPER XII

Category:C

Year & Semester

Third year & Fifth semester

Credits

4


Subject code

Objectives

To gain knowledge about biochemical estimations and hematological techniques

Course content: Practical III (90 HRS)
1. Colorimetric Estimation

a. Estimation of creatinine by Jaffe’s method

b. Estimation of urea by diacetyl monoxime method.

c. Estimation of DNA.

d. Estimation of RNA.

e. Estimation of calcium in blood



2. Experiments on Clinically important enzymes by colorimetry

a. Effects of pH, temperature and substrate concentration for amylase.

b. Assay of serum amino transferases (SGOT and SGPT). and LDH.

3. Hematology

RBC count, PCV, ESR, Total and differential WBC count.



Reference Books:

Varley’s Practical Clinical Biochemistry by Alan H Gowenlock, published by CBS Publishers and distributors, India Sixth Edition (1988).

Tietz Fundamentals of Clinical chemistry – Burtis and Ashwood, Fifth Edition, WB Saunders Company, Oxford Science Publications USA, (2001)

Practical Biochemistry – Keith Wilson & John Walker, fifth edition, Cambridge Universtiy Press, UK (2000).

Analytical Biochemistry & Separation Techniques – Palanivelu third edition (2004).

Biochemical calculations – Irwin H. Segel, second edition, John Wiley & Sons, USA (1976).

Introduction to Practical Biochemistry by Sawhney and Randhir Singh, Narosa Publishing House, London (2000).

Experiments in Biochemistry – A Hands on Approach by Shawn O Farrell and Ryan T Ranallo, Thomson Learning Inc., USA (2000)



Recommended Books:

Principles of Instrumental analysis – Skoog, Holler, Nieman, fifth edition Published by Harcourt College Publishers, Singapore (2001).

Essentials of Practical Biochemistry by Lalit M Srivatsava, Nibhriti Das, Subrata Sinha, Published by CBS Publishers and Distributors, India, (2002)
Indian Books:

Analytical Biochemistry & Separation Techniques – Palanivelu third edition (2004).





Title of the paper

BIOTECHNOLOGY –ELECTIVE 1

Category: E

Year & Semester

Third year & Fifth semester

Credits

5


Subject code

Objectives

To gain knowledge about basic biotechnology


Course Contents : Biotechnology (90 HRS)
UNIT I

Biotechnology- scope, features and importance of biotechnology, Recombinant DNA technology- role of restriction endonucleases, plasmid and cosmid cloning vectors. Brief outline of molecular cloning- genomic DNA libraries, cDNA, PCR, applications of recombinant DNA technology. (18 hrs)


UNIT II

Fermentors- principle, design, mode of operation, instrumentation and control product recovery and purification. Production of ethanol. Microbial, algal technology- single cell proteins, cultivation of spirullina. (18 hrs)


UNIT III

Renewable sources of energy and fuel using microorganisms- chemical and biological conversion, sewage treatment using microbial systems, biofertilizers, biopesticides. (18 hrs)


UNIT IV

Plant genome organization – structure of representative plant genes and gene families in plants – organization of chloroplast genome – organization of mitochondrial genome – plant tissue culture – suspension cultured cells – haploid plants – cloning of hosts – microbe propagation – somatic embryogenesis – protoplast isolation and applications. (18 hrs)


UNIT V

Historical aspects of medical biotechnology – pathogenic microbes – bacterial, viral, fungal and protozoan diseases – diagnosis using modern techniques – probes – cure, control and prevention. Health – disease diagnosis – Hybridoma technique, Monoclonal antibodies – application of probes for diagnosis of existing and emerging diseases in animal and human disease. (18 hrs)


Reference Books:

Jogdand, S.N. 2000. Medical Biotechnology Himalaya Publishing House, Mumbai.

Click B.R. and Pasternark. 2002. Molecular Bio-technology principle and applications of recombinant DNA. ASM press.

In Biotechnology, University of Madras of publications, Pearl Press.

Trevan. 2001. Biotechnology. Tata Mc Graw – Hill.

Industrial Biotechnology – Casida et al,1964

Jenkins, N (ed) 1999. Animal cell biotechnology: methods and protocols. Humana press, New Jersey, pp 1-302.

Watson, J.D. Hopkins, NH, Roberts, J.W. Steitz, J.A. and Weiner A.M. 1987. Molecular Biology of Gene. Benjamin / Cummings 4th ed. Vol. 1&2.



Recommended Books:

Walker, J.M. and Gingold, E.B. 1999. Molecular biology and Biotechnology, 3rd edition. Panima publishing corporation.

Puhler A.V.C.H.1993. Genetic engineering of animals. VCH publishers. Weinbuin, FRG

Watson, J.D. Gillman, M. Witknowski, J and Zoller, M. 1992. Recombinant DNA (2nd edn) scientific American books. NY.

Murray, E.T. 1991. Gene transfer and expression protocols – Methods in molecular biology. Volume 7. Humana press.

Genes VIII – Benjamin Lewin, 2004, 1st edition, Published by Pearson Prentice Hall, Pearson Education Inc.


Indian Books:

Ignacimuthu. 1996. Basic biotechnology. Tata Mc Graw – Hill.



Web Sites:

www.sciencedirect.com

SEMESTER- VI


Title of the paper

NUTRITIONAL BIOCHEMISTRY AND HUMAN RIGHTS –PAPER X

Category:C

Year & Semester

Third year & Sixth semester

Credits

5


Subject code

Objectives

To gain knowledge about the dietary patterns and insight about the human rights


Course Content: Nutritional Biochemistry and Human Rights (90 HRS)
UNIT I

Introduction and definition of food and nutrition. Basic food groups- energy yielding, body building and protective foods. Basic concepts of energy expenditure, unit of energy, measurement of food stuffs by Bomb calorimeter. Basal metabolism – definition, BMR, factors affecting BMR. Calorific value of proteins, carbohydrates and fats, RQ of foods. SDA- definition and determination. (18 hrs)


UNIT II

Nutritive value and physiological role of carbohydrates and lipids . Nutritive value of proteins, essential amino acids, essential fatty acids, biological value of animal and plant proteins. Evaluation of proteins by nitrogen balance method – DC, BV, NPU and NAP of animal and plant proteins, protein sparing action of carbohydrates. Protein malnutrition (Kwashiorkor) and under nutrition (Marasmus), their preventive and curative measures. (18 hrs)


UNIT III

Composition of balanced diet and RDA for infants, children, adolescents, adult male and female, pregnant, lactating women and old age. Diet for disease states- Diabetes mellitus, Hyper tension, Atherosclerosis, Jaundice, Pectic ulcer

(18 hrs)

UNIT IV

Source, dietary allowance, biochemical role, physiological role and deficiency state. (18 hrs)


UNIT V

Human rights – introduction- definition, scope and need for study of human rights and relations. Categories- civil and political rights, economic relations and social relations. Institutions : International and National- United Nations human right commission, State human rights commission. Instruments- VDHR, International Convention on civil and political rights. International convention on economic and social rights. National human rights act- National Commision for minorities SC/ST and Women Students activity- assignment, case study, term paper. (18 hrs)


Reference Books:

Techniques of Food Analysis – Andrew L Winton & Kate Barber Winton, 2001, Published by Agrobios (India) Ltd.

Human Nutrition and Dietetics – Garrow & James, 1996, 9th edition, published by Chruchill Livingstone Inc.

Antioxidant status, diet, nutrition and health – Andreas M Pappas, 1996, published by CRC Press, Washington, DC.

Human rights and social movements. Anmol publishers, Aravind Kumar(ed) 1999.

Human rights Under the Indian Constitution, P.L. Mehta, Meena Urma 1999.

National Human Rights Commission of India, Arun kumar Palai, Atlantic publishers- 1999.

Recommended Books:

Nutritional Biochemistry – Tom Brody, 1994, Academic Press, USA.

Food Fundamentals – Margaret Mc Williams, 2nd edition.
Indian Books:

Essentials of food and nutrition – Swaminathan. M, Vol 1, 1995, Vol-2,1974




Title of the paper

CLINICAL BIOCHEMISTRY – PAPER XI

Category:C

Year & Semester

Third year & sixth semester

Credits

5


Subject code

Objectives

To gain knowledge about the metabolic disorders


Course Contents : Clinical Biochemistry (90 HRS)
UNIT I

Blood glucose relation, hypo and hyperglycemia. Diabetes mellitus – types, clinical features and metabolic changes. Glucosuria, galactosemia and fructosuria. Glycogen storage diseases- symptoms, diagnosis, biochemical changes and treatment. (18 hrs)



UNIT II

Etiology and clinical manifestation of phenyl ketonuria, cystinuria, alkaptonuria, Fanconi’s syndrome, albinism and tyrosinemia, hypo and hyperuricemia, Gout. Serum lipids in diseases with special reference to cholesterol, lipidosis, triglyceridemia, hypo and hypercholesterolemia. Clinical features ofatherosclerosis and fatty liver. (18 hrs)



UNIT III

Liver Function Tests – Metabolism of bilirubin, Jaundice – types, clinical features and test based on bile pigments level in blood and urine, Plasma changes, PT, Differentiation of three types of jaundice.

Gastric Function Tests – Collection of gastric contents, examination of gastric residium, FTM, stimulation tests, tubeless gastric analysis. (18 hrs)
UNIT IV

Renal Function Tests – Clearance tests – Urea, Creatinine, inulin, PAH test, concentration and dilution test.

Tumor Markers – Definition, markers produced by various tissues, classification and clinical applications. (18 hrs)
UNIT V

Clinical Enzymology – Definition of functional and non-functional plasma enzymes. Isozymes and diagnostic tests, enzyme patterns in acute pancreatitis, liver damage, bone disorders, myocardial infarction and muscle wasting. (18 hrs)
Reference Books:

Varley's Practical Clinical Biochemistry - Alan H Gowenlock, published by CBS Publishers and distributors, 1988, 6th edition, India.

Textbook of Biochemistry with clinical correlations-T.M.Devlin, 2002, 5th edition.

Biochemistry: A case oriented approach- Montgomery, Comway, Spector, Chappell,1996, 6th edition, Mosby Publishers, USA.



Recommended Books:
Tietz Fundamentals of Clinical chemistry – Burtis and Ashwood, 2001, 5th edition, WB Saunders Company, Oxford Science Publications USA.
Indian Books:

Text book of Biochemistry : A clinically oriented approach - Dinesh Puri, B.I, 2002, Churchill Livingstone Inc., India.

Text Book of Medical Biochemistry – Chatterjea & Schinde, 2000 4th edition.

Title of the paper

PRACTICAL IV – PAPER XIII

Category:C

Year & Semester

Third year & sixth semester

Credits

4


Subject code

Objectives

To gain knowledge about the enzyme assays


Course Content : Practical IV (90 HRS)

1. Colorimetric Estimation

a. Estimation of glucose by O-toluidine method.

b. Estimation of albumin and A/G ratio in serum by Biuret method.

c. Estimation of cholesterol by Zak’s method.



2. Experiments on enzymes by colorimetry

a. Effects of pH, temperature and substrate concentration for urease.

b. Assay of activity of Alkaline phosphatase in serum.

3. Urine and Faeces analysis

a. Collection of urine and faecal samples.

b. Faecal analysis to detect fats, undigested food and blood.

c. Qualitative analysis of urine for normal and pathological conditions.

d. Estimation of urine urea and creatinine.

Reference Books:

Varley’s Practical Clinical Biochemistry by Alan H Gowenlock, published by CBS Publishers and distributors, India Sixth Edition (1988).

Tietz Fundamentals of Clinical chemistry – Burtis and Ashwood, Fifth Edition, WB Saunders Company, Oxford Science Publications USA, (2001)

Practical Biochemistry – Keith Wilson & John Walker, fifth edition, Cambridge Universtiy Press, UK (2000).

Analytical Biochemistry & Separation Techniques – Palanivelu third edition (2004).

Biochemical calculations – Irwin H. Segel, second edition, John Wiley & Sons, USA (1976).

Introduction to Practical Biochemistry by Sawhney and Randhir Singh, Narosa Publishing House, London (2000).

Experiments in Biochemistry – A Hands on Approach by Shawn O Farrell and Ryan T Ranallo, Thomson Learning Inc., USA (2000)


Recommended Books:

Principles of Instrumental analysis – Skoog, Holler, Nieman, fifth edition Published by Harcourt College Publishers, Singapore (2001).

Essentials of Practical Biochemistry by Lalit M Srivatsava, Nibhriti Das, Subrata Sinha, Published by CBS Publishers and Distributors, India, (2002)
Indian Books:

Analytical Biochemistry & Separation Techniques – Palanivelu third edition (2004).



Title of the paper

IMMUNOLOGY - ELECTIVE II

Category:E

Year & Semester

Third year & sixth semester

Credits

5


Subject code

Objectives

To gain knowledge about immunity and immunological techniques


Course Contents: Immunology (90 HRS)
UNIT I

Immunity and its types. Innate immunity, determinants of innate immunity, peptides, Acquired immunity, active and passive immunity. Commonly used toxoid vaccines, killed vaccines, live attenuated vaccines, bacterial polysaccharide vaccines. (18 hrs)



UNIT II

Immunity – Humoral and Cellular immunity, Immunoglobins – structure and function, types of antibodies. (18 hrs)

UNIT III

Antigens – Nature, immunogens, haptens. Cells involved in antibody formation, differentiation of lymphocyte, clonal selection theory, co-operation of T-cell with B-cell, secretion of antibody, genetic basis of antibody diversity. (18 hrs)
UNIT IV

Antigen-antibody reactions in vivo and in vitro. Complement fixation reaction, monoclonal antibody – preparation and application in biology. (18 hrs)


UNIT V

Immunological tolerances and immunosuppression, hypersensitivity and allergy, histocompatibility antigens – elementary knowledge, auto-immune diseases,

transplantation immunology. (18 hrs)
Reference Books:

Immunology – Janis Kuby, 2000, 4th edition, WH Freeman Company, USA.

Essential Immunology – Ivan Roitt, 1997, Blackwell Science Publishers, UK.

Recommended Books:

A Hand Book of Practical Immunology – Talwar G.P, 1983, Vikas Publishing House.

Immunology – Weir D.M., 8th edition 1997.

Indian Books:

An introduction to Immunology – Rao C.V, 2002, 1st edition, Narosa Publishers, India.



Web Sites:

www.ebi.ac.uk/2can/tutprials/nucleotide/vector.html

www/nucleotide/blast/html.com



http://www.cato.com/biotech/bio-info.html


Title of the paper

PHYSIOLOGY – ELECTIVE III

Category: E

Year & Semester

Third year & sixth semester

Credits

5


Subject code

Objectives

To gain knowledge about organ physiology
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