The balancing act. Redox poise and signalling The Drummond lecture



Download 13,93 Kb.
Date conversion27.11.2017
Size13,93 Kb.
  • New Zealand
  • Dr Muriel Emma Bell (CBE) was awarded the William Gibson Research Scholarship for Medical Women of the British Empire by the Royal Society of Medicine. This enabled her to work for three years with Professor Jack Drummond at University College, London.
  • Australia
  • Australian Academy of Science. Victor Martin Trikojus 1902-1985 went to London, where Trik spent some months with Professor Jack Drummond at University College. This gave him the opportunity of extending his contacts with endocrinologists, as well as getting to know one of the great figures in British nutrition.
  • Wales….
  • David Hughes, the first Professor and Head of Department of Microbiology at Cardiff (1964-1982) brought back happy memories of the golden past. He also brought the house down with his story of Sir Jack Drummond who coated a haddock with yellow phosphorus to demonstrate to medical students, its "bioluminescence". Trouble was his hands glowed in the darkened lecture theatre more strongly than the fish!
  • David Lloyd, Biotype. The Newsletter of the School of Pure & Applied Biology, University of Wales Cardiff. Volume 6 Issue 2 April 1996
  • Acknowledgements
  • Lund University Plant Cell Biology-Plant Biochemistry http://plantcell.lu.se
  • Thomas Pfannschmidt (Now University of Jena, Germany)
  • Dalibor Stys (Now S. Bohemia University, Czech Republic)
  • Anders Nilsson (Now Bruker Spectroscopy, Lund)
  • Sylvan Dubé (Now Laval University, Canada)
  • Paul Davison (Now Sheffield University, U.K.)
  • H. Louise Race (Now Aylesbury, U.K.)
  • Jens Forsberg
  • Jörgen Ström
  • Boaz Laadan
  • Carol Allen
  • Collaborations
  • Torbjörn Drakenberg, Sture Forsén (Lund Physical Chemistry 2)
  • Gerhard Link (University of Bochum, Germany)
  • Light 2
  • Light 1

The balancing act 1.

  • Transients, enhancement, and redox poise of cyclic electron flow.
  • Rates and activities of components.
  • Myers, J. (1971) Annu. Rev.Plant Physiol. 22, 289-312
  • Hill, R. (1965) Essays Biochem. 1, 121-151
  • Park, R. B. and Sane, P.V. (1971) Annu. Rev. Plant Physiol.22, 395-430
  • Photosynthesis
  • Blankenship, R. E. (2002) Molecular Mechanisms of Photosynthesis. Blackwell Science, Oxford
  • Heathcote, P. et el. (2002) Trends Biochem. Sci. 27, 79-87
  • Forsberg, J. (2002) PhD thesis. Lund University
  • Pfannschmidt, T. et al. (1999) IUBMB Life 48, 271-276

The balancing act 2.

  • Protein phosphorylation and state transitions. Post-translational modification of the structure and function of proteins you already have.
  • Allen, J. F. (1992) BBA 1098, 275-225 . Adapted from Allen, J.F. et al. (1981) Nature 291, 25-29
  • Dubé, S., Davison, P.A. and Allen, J. F. (unpublished)
  • State 1
  • State 2
  • State 1
  • State 2
  • Allen, J. F. and Forsberg, J. (2001) Trends Plant Sci.6, 317-326
  • Nilsson, A. et al. (1997) JBC 272, 18350-18357

The balancing act 3.

  • Redox control of gene expression. Changing transcription and the stoichiometry of complexes.
  • Allen, J. F. (1992) BBA 1098, 275-225
  • Pfannschmidt, T. et al. (1999) IUBMB Life 48, 271-276

The balancing act 4.

  • Redox signalling and evolution.
  • Why chloroplasts (and mitochondria…) have genomes
      • Race, H. L., Herrmann, R. G. and Martin, W. (1999) Trends Genet. 15, 364-370
  • Allen, J. F. (1993) J. Theor. Biol. 165, 609-631
  • GENE
  • PROTEIN
  • SUBSTRATE
  • Photosystem II
  • psbA
  • +1,170 (P+/P)
  • -800 (P/P*)
  • Light
  • psbD
  • D2 of PS II reaction centre
  • +1,170 (P+/P)
  • -800 (P/P*)
  • Light
  • psbB, psbC
  • CP47 and CP43
  • of PS II
  • -
  • Light
  • petA
  • Cytochrome f
  • +350
  • Pmf (pH)
  • petB
  • Cytochrome b6
  • -50
  • Pmf (pH)
  • atpA,B,E
  • ATP synthase CF1,,
  • pH, ATP
  • atpF,H,I
  • ATP synthase CFoI,III,IV
  • -
  • pH, ATP
  • Photosystem I
  • psaA
  • -1,250 (P/P*)
  • +500 (P+/P)
  • Light
  • psaB
  • A2 of PS I reaction centre
  • -1,250 (P/P*)
  • +500 (P+/P)
  • Light
  • Respiration; cyclic electron transport
  • ndhA-F
  • NADH dehydrogenase subunits 1-5
  • -324
  • Respiratory substrates?
  • rbcL
  • Rubisco large subunit
  • -
  • Carbon dioxide
  • Allen, J. F. (1993) J. Theor. Biol. 165, 609-631

The balancing act. Redox poise and signalling

  • Transients, enhancement, and redox poise of cyclic electron flow.
  • Protein phosphorylation and state transitions.
  • Redox control of gene expression.
  • Redox signalling and evolution.

Quinone pools: Why is redox poise maintained, apparently by every possible means?

  • Efficiency. Example - state transitions in oxygenic photosynthesis.
  • Safety - minimizing single electron transfers to oxygen.

The balancing act Postscript.

  • The Q-cycle, quinone and superoxide anion radicals. The mitochondrial theory of ageing, and the evolution of sex.
  • GENE
  • PROTEIN
  • STANDARD REDOX POTENTIAL, Em7 (mV)
  • SUBSTRATE
  • Electron transport
  • nad1-7
  • Seven subunits of NADH dehydrogenase
  • -324
  • cox1-3
  • Cytochrome c oxidase subunits I, II and III
  • +815
  • Oxygen
  • Energy coupling
  • atp6
  • ATP synthase F1 subunit 6
  • -
  • Proton motive force, ATP
  • cob
  • Cytochrome b
  • -50
  • Proton motive force
  • Allen, J. F. (1993) J. Theor. Biol. 165, 609-631
  • Mitochondrial genes and gene products
  • Crofts, A.R., et al. (1998) In Proceedings of the IXth. International Symposium on Phototrophic Prokaryotes, Vienna, (Peschek G., ed.)
  • ALLEN, J F (1996) J. Theor. Biol. 180, 135-140
  • Thank you for your attention
  • The balancing act. Redox poise and signalling
  • The Drummond lecture
  • Queen Mary, University of London
  • http://plantcell.lu.se/balancing
  • ALLEN, J F (1996) J. Theor. Biol. 180, 135-140


The database is protected by copyright ©sckool.org 2016
send message

    Main page